01H9-derived | model identity
E-cadherin, LRP2 and Nephrin profiling of nephron-associated identity
E-cadherin, LRP2 and Nephrin immunofluorescence profile cell types in H9-derived kidney organoids.
Study resultsSafety case study
Kidney organoids for safety and injury research
Investigate drug-related kidney injury through cisplatin-associated molecular changes and time-course imaging after doxorubicin exposure.
Model & sampleH9-derived kidney organoids and human kidney organoid injury studies
Study setupCisplatin molecular readouts in H9-derived kidney organoids and doxorubicin acute-injury observations in human kidney organoids
AssaysE-cadherin, LRP2, Nephrin, RT-qPCR, serial morphology and study-specific injury endpoints
STUDY EVIDENCE
Kidney organoid approaches for identity and injury studies
Cisplatin molecular readouts in H9-derived organoids and doxorubicin morphology from a separate human kidney study.
Study design details
01Model identityEstablish nephron-associated identity
02Exposure designDefine dose, timing and controls
03Multimodal assaysConnect morphology, IF and molecular endpoints
04Follow-on workSelect mechanism and recovery studies
H9-derived approach: identity and cisplatin molecular readouts2 study figures
02H9-derived | cisplatin molecular readouts
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KIM-1, H2AFX, inflammation and stress readouts
RT-qPCR records KIM-1, H2AFX, IL-8, IL-1β and PUMA expression after cisplatin exposure.
Human kidney study: doxorubicin acute-injury observation1 study figure
03Injury study | serial morphology
Representative fields before and 24/48 hours after exposure
Bright-field images before and 24 and 48 hours after doxorubicin exposure show changes in kidney organoid morphology.
Study application
Cisplatin molecular readouts in H9-derived organoids and doxorubicin morphology in a separate human kidney study provide complementary molecular and structural views of kidney injury.
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PROJECT DISCUSSION
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