Barrier and delivery question
Define tissue interface, candidate format and whether the question is transport, retention, injury or repair.
Application
Organoid delivery and barrier studies
Start with the target tissue’s barrier structure, develop permeability readouts for the project, then study candidate transport or retention.
Human intestinal organoid study
Observe barrier change, then design the delivery study
An IBD-like inflammation study shows a FITC-Dextran barrier readout and IL-8 changes. Candidate transport requires separate tracer, recovery and barrier-integrity assays; BBB studies select the cell system and functional readouts for the project.
View imageBarrier and inflammatory readouts | FITC-positive area and mean IL-8 rose under inflammatory conditions. FITC uses matched representative fields; IL-8 has three measurements per condition.
Study data
View imageCandidate image readout | Relative area: vehicle 68%, tofacitinib 112%, candidate 111%. Each arm is normalized to its own baseline in a representative field.
Study dataReview the full study
View the IBD model and barrier study Continue to the related case for model context, experimental setup and interpretation.
Study focus
Questions we can help answer
- 01What functional baseline does the barrier model achieve?
- 02How can candidate transport or retention be quantified reliably?
- 03How does permeability change relate to barrier injury?
Study workflow
From study goal to results
Baseline and batch QC
Confirm cell identity, tight junctions, baseline permeability and acceptance criteria.
Exposure and permeability
Define orientation, time, concentration, recovery and controls with appropriate tracing or quantification.
Transport and barrier integrity
Interpret permeability with viability, morphology and junction markers to build an integrated view of transport, retention and barrier status.
Model options
Models for your study
Intestinal organoids support barrier-function and inflammation studies, while blood–brain barrier programs use co-development to configure the cell system and functional endpoints. Permeability can be interpreted with recovery, mass balance and barrier integrity to guide follow-on in vivo work.
PROJECT DISCUSSION
Plan a delivery or barrier study
Tell us about the candidate or formulation, the target barrier and the exposure direction. We’ll recommend the model and the permeability and barrier-integrity readouts needed to assess transport, retention or barrier effects.