Phenotype and groups
Define the reliable functional phenotype, then group by response, model feature, dose or time.
Application
Disease mechanism & biomarkers
Compare tissue images, functional assays and molecular results across models or treatments to identify biomarker candidates for follow-up validation.
Two separate studies
Find leads in tissue features and treatment response
An HCC study shows source-tissue and PDO histology and marker staining; a separate kidney organoid study shows molecular changes after cisplatin exposure. A candidate marker can then be tested in additional samples relevant to your program.
View imageHCC tissue and model profiling: H&E histology and figure-labelled CD31 and HEP-1 immunohistochemistry in source tissue and PDO provide tissue-architecture and marker-staining context for subsequent target and mechanism studies.
Model & assay data
View imageCisplatin-associated molecular changes: RT-qPCR compares KIM-1, H2AFX and inflammation- and stress-related gene expression in H9-derived kidney organoids.
TRiCBIO H9-derived kidney organoid cisplatin studyReview the full study
Continue to the related case for model context, experimental setup and interpretation.
Study focus
Questions we can help answer
- 01Which pathway changes track with a reliable phenotype?
- 02Which baseline features best distinguish response?
- 03Which candidate markers are reproducible across independent samples and orthogonal methods?
Study workflow
From study goal to results
Mechanism-focused assays
Select histology, imaging, flow, qPCR, protein or secreted readouts around the hypothesis.
Data integration and candidate markers
Compare baseline, treatment response and mechanism readouts to identify reproducible candidate markers.
Independent validation and evidence building
Validate in additional samples or with orthogonal methods, strengthening the evidence from initial observation through independent confirmation.
Model options
Models for your study
Identify candidate signals associated with treatment response or mechanism, then build a validation plan using additional samples, prespecified analyses and orthogonal methods.
PROJECT DISCUSSION
Plan a mechanism or biomarker study
Disease context, existing phenotypes and data, comparison groups and the mechanism hypothesis shape the models, assays and complementary confirmation plan.