Target and tissue context
Use histology, IHC or IF to assess target expression and spatial heterogeneity, and confirm model identity and culture quality.
Application
ADC efficacy & tissue distribution
Compare ADC candidates in human organoids using target-expression, tissue-distribution and activity readouts, with dedicated studies of bystander killing.
Breast PDO study images
See distribution and ADC activity in separate studies
One frozen-section study shows tissue distribution of AF549-labeled antibody and nanobody formats. A separate ADC study shows morphology and viability after treatment. We can design distribution imaging, activity and dedicated penetration assays for your candidate.
View imageFrozen-section spatial distribution: bright-field, DAPI, AF549 and merged views compare relative signal patterns for labeled antibody and nanobody in representative PDO frozen sections.
TRiCBIO breast cancer PDO antibody/nanobody distribution study
View imagePost-treatment morphology: bright-field views of the control and two ADC treatment arms provide tissue context for viability readouts.
TRiCBIO breast cancer PDO ADC study data
View imageADC dose response: two breast cancer PDO models show distinct viability profiles across candidate ADC concentrations.
TRiCBIO breast cancer PDO ADC study dataReview the full study
View antibody distribution and ADC response Continue to the related case for model context, experimental setup and interpretation.
Study focus
Questions we can help answer
- 01How do target level and spatial pattern support candidate comparison?
- 02How do tissue distribution and killing relate?
- 03When are penetration, bystander or immune endpoints valuable?
Study workflow
From study goal to results
Labeling and spatial imaging
Define labeling, incubation, imaging and competition or target-negative controls around molecule properties; add dedicated imaging and quantification when intact-organoid penetration is a priority.
Killing and mechanism endpoints
Interpret spatial distribution with dose response, viability, apoptosis and histology.
ADC ranking
Compare candidates consistently and deliver original images, quantitative analysis and interpretation.
Model options
Models for your study
ADC studies first compare candidates through section imaging and functional readouts; quantitative penetration studies can then add serial sections or z-stacks, distance/intensity profiles, time points and target/competition controls.
PROJECT DISCUSSION
Plan an ADC organoid study
ADC design and target biology determine the tumor models, distribution imaging, killing and bystander endpoints for the study.